Nano Letters Paper on Rapid RNA Quantification in Lipid Nanoparticles

Rapid and Accurate Quantification of RNA in Lipid Nanoparticles by Scatter-Free UV/Visible Spectroscopy

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Marama Labs is pleased to highlight a new peer-reviewed publication in Nano Letters, developed in collaboration with CureVac, demonstrating a rapid and accurate method for quantifying RNA directly in intact lipid nanoparticles (LNPs) using Scatter-Free UV/Visible Spectroscopy.

The study addresses a key limitation in conventional UV/Vis analysis, where light scattering from LNPs prevents reliable RNA measurement. It also highlights thechallenges of fluorescence-based assays such as RiboGreen, which require extensive sample preparation and can show limited precision.

The authors present Scatter-Free Absorption Spectroscopy (SFAS), an integrating sphere-based approach that isolates true absorption from scattering, enabling direct RNA quantification in intact LNP formulations.

Key Insights

Thestudy demonstrates that SFAS enables:

• Direct RNA quantification in intact LNPs without disruption or extraction
• Eliminationof scattering artefacts that limit conventional UV/Vis methods
• High precision (∼1.5%) and estimated accuracy (∼5%)
• Rapid, low-preparation workflows compared to fluorescence assays
• Strong linearity and reproducibility across multiple LNP formulations

Read the Full Publication

Explore the full study in Nano Letters to learn more about the method and validation results.

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