Applications
Just like a regular UV-Vis, there are lots of applications for CloudSpec - very probably many we haven't thought of yet. If your sample scatters, but you could otherwise analyse it by UV/Vis, then CloudSpec might be perfect for you.
Get in touch if you have a cloudy sample we haven't talked about below.
Drug Loading of Nanoparticles
Drug loading of nanoparticles is often exceedingly difficult to quantify, relying on tedious fluorescence assays or complicated sample preparation. CloudSpec can quantify drug loading in seconds with only a simple dilution required.
Quantifying RNA in Lipid Nanoparticles (LNPs) is trivial with CloudSpec. The highly scattering UV spectrum of a loaded LNP becomes a straightforward A260 analysis of RNA content.
Metal Nanoparticles
UV-Vis is a common tool for particle size, concentration and batch consistency, but it's limited in analysis because only the extinction of the particles is measured. Also, as particle size increases the scatter tends to overwhelm the spectrum, obscuring the important absorption features.
CloudSpec’s extinction, absorption and scattering spectra yield much more information about the sample, including improved particle size, particle number density and particle engineering.
Bacterial and Cell Growth Monitoring
UV/Vis is ideal for biological samples, protein and DNA/RNA concentrations, chromophore analysis and more. Because most biological solutions, such as cell cultures, are inherently turbid due to light scattering from cells, aggregates and other biological components, spinning down of samples is often required to clarify the liquid.
CloudSpec can measure raw cultures and even live cells directly without centrifuging the sample. With SFA measurements you can measure the whole content of the sample at the same time without any separation.
